diff --git a/Modules/Core/Common/wrapping/test/itkImageInteropTest.py b/Modules/Core/Common/wrapping/test/itkImageInteropTest.py
index 8e341621408..b63c07ce802 100755
--- a/Modules/Core/Common/wrapping/test/itkImageInteropTest.py
+++ b/Modules/Core/Common/wrapping/test/itkImageInteropTest.py
@@ -1,4 +1,4 @@
-#!/usr/bin/env python
+#!/usr/bin/env python3
 # ==========================================================================
 #
 #   Copyright NumFOCUS
diff --git a/Modules/Filtering/BinaryMathematicalMorphology/wrapping/test/BinaryDilateImageFilterTest.py b/Modules/Filtering/BinaryMathematicalMorphology/wrapping/test/BinaryDilateImageFilterTest.py
index 731e3ddd9a9..611048b3591 100755
--- a/Modules/Filtering/BinaryMathematicalMorphology/wrapping/test/BinaryDilateImageFilterTest.py
+++ b/Modules/Filtering/BinaryMathematicalMorphology/wrapping/test/BinaryDilateImageFilterTest.py
@@ -1,4 +1,4 @@
-#!/usr/bin/env python
+#!/usr/bin/env python3
 
 # ==========================================================================
 #
diff --git a/Modules/Filtering/BinaryMathematicalMorphology/wrapping/test/BinaryErodeImageFilterTest.py b/Modules/Filtering/BinaryMathematicalMorphology/wrapping/test/BinaryErodeImageFilterTest.py
index 4d77726302a..c9a7965ca61 100755
--- a/Modules/Filtering/BinaryMathematicalMorphology/wrapping/test/BinaryErodeImageFilterTest.py
+++ b/Modules/Filtering/BinaryMathematicalMorphology/wrapping/test/BinaryErodeImageFilterTest.py
@@ -1,4 +1,4 @@
-#!/usr/bin/env python
+#!/usr/bin/env python3
 
 # ==========================================================================
 #
diff --git a/Modules/Filtering/MathematicalMorphology/wrapping/test/GrayscaleDilateImageFilterTest.py b/Modules/Filtering/MathematicalMorphology/wrapping/test/GrayscaleDilateImageFilterTest.py
index 139e7a80f03..716f46762fc 100755
--- a/Modules/Filtering/MathematicalMorphology/wrapping/test/GrayscaleDilateImageFilterTest.py
+++ b/Modules/Filtering/MathematicalMorphology/wrapping/test/GrayscaleDilateImageFilterTest.py
@@ -1,4 +1,4 @@
-#!/usr/bin/env python
+#!/usr/bin/env python3
 
 # ==========================================================================
 #
diff --git a/Modules/Filtering/MathematicalMorphology/wrapping/test/GrayscaleErodeImageFilterTest.py b/Modules/Filtering/MathematicalMorphology/wrapping/test/GrayscaleErodeImageFilterTest.py
index 39f9febc5ac..0b58fcdb1f3 100755
--- a/Modules/Filtering/MathematicalMorphology/wrapping/test/GrayscaleErodeImageFilterTest.py
+++ b/Modules/Filtering/MathematicalMorphology/wrapping/test/GrayscaleErodeImageFilterTest.py
@@ -1,4 +1,4 @@
-#!/usr/bin/env python
+#!/usr/bin/env python3
 
 # ==========================================================================
 #
diff --git a/Utilities/Doxygen/datetime.py b/Utilities/Doxygen/datetime.py
index 7552851e48b..8801fbed1aa 100755
--- a/Utilities/Doxygen/datetime.py
+++ b/Utilities/Doxygen/datetime.py
@@ -1,4 +1,4 @@
-#!/usr/bin/env python
+#!/usr/bin/env python3
 #
 #  Copyright NumFOCUS
 #
diff --git a/Utilities/Doxygen/mcdoc.py b/Utilities/Doxygen/mcdoc.py
index efd37cb2b0c..eb2c2222684 100755
--- a/Utilities/Doxygen/mcdoc.py
+++ b/Utilities/Doxygen/mcdoc.py
@@ -1,4 +1,4 @@
-#!/usr/bin/env python
+#!/usr/bin/env python3
 
 import sys, os, re, glob
 
diff --git a/Utilities/ITKv5Preparation/EnumPrintFunction.py b/Utilities/ITKv5Preparation/EnumPrintFunction.py
index 26ed6dd9c72..7a4327e07ef 100755
--- a/Utilities/ITKv5Preparation/EnumPrintFunction.py
+++ b/Utilities/ITKv5Preparation/EnumPrintFunction.py
@@ -1,4 +1,4 @@
-#!/usr/bin/env python
+#!/usr/bin/env python3
 # \author Mathew J. Seng
 # This python script helps transition existing enumerations to ITKv5's strongly typed enumerations.
 
diff --git a/Utilities/ITKv5Preparation/replaceClassWithTypename.py b/Utilities/ITKv5Preparation/replaceClassWithTypename.py
index ff1eb1da80c..9268f607b79 100755
--- a/Utilities/ITKv5Preparation/replaceClassWithTypename.py
+++ b/Utilities/ITKv5Preparation/replaceClassWithTypename.py
@@ -1,4 +1,4 @@
-#!/usr/bin/env python
+#!/usr/bin/env python3
 # \author Hans J. Johnson
 # The following python script was used to make the ITK code consistent.
 
diff --git a/Utilities/Maintenance/ArchiveTestingDataOnAzure.py b/Utilities/Maintenance/ArchiveTestingDataOnAzure.py
index 43f4fc10201..cfda163a034 100755
--- a/Utilities/Maintenance/ArchiveTestingDataOnAzure.py
+++ b/Utilities/Maintenance/ArchiveTestingDataOnAzure.py
@@ -1,4 +1,4 @@
-#!/usr/bin/env python
+#!/usr/bin/env python3
 
 
 description = """
diff --git a/Utilities/Maintenance/BuildHeaderTest.py b/Utilities/Maintenance/BuildHeaderTest.py
index df34640bfd9..036e8ed948f 100755
--- a/Utilities/Maintenance/BuildHeaderTest.py
+++ b/Utilities/Maintenance/BuildHeaderTest.py
@@ -1,4 +1,4 @@
-#!/usr/bin/env python
+#!/usr/bin/env python3
 
 # ==========================================================================
 #
diff --git a/Utilities/Maintenance/FindRedundantHeaderIncludes.py b/Utilities/Maintenance/FindRedundantHeaderIncludes.py
index 591863fcd6c..97eaf0de3f8 100755
--- a/Utilities/Maintenance/FindRedundantHeaderIncludes.py
+++ b/Utilities/Maintenance/FindRedundantHeaderIncludes.py
@@ -1,4 +1,4 @@
-#!/usr/bin/python
+#!/usr/bin/env python3
 # ==========================================================================
 #
 #   Copyright NumFOCUS
diff --git a/Utilities/Maintenance/JREUpdate.py b/Utilities/Maintenance/JREUpdate.py
index 72c4ce0de9b..5f898d565b3 100755
--- a/Utilities/Maintenance/JREUpdate.py
+++ b/Utilities/Maintenance/JREUpdate.py
@@ -1,4 +1,4 @@
-#!/usr/bin/env python
+#!/usr/bin/env python3
 
 description = """
 Update the JRE tarballs to be bundled with the SCIFIOImageIO plugin.
diff --git a/Utilities/Maintenance/UpdateCopyrightStatementsInITK.py b/Utilities/Maintenance/UpdateCopyrightStatementsInITK.py
index 49871beee9f..a67a719c75e 100755
--- a/Utilities/Maintenance/UpdateCopyrightStatementsInITK.py
+++ b/Utilities/Maintenance/UpdateCopyrightStatementsInITK.py
@@ -1,4 +1,4 @@
-#!/usr/bin/python
+#!/usr/bin/env python3
 # ==========================================================================
 #
 #   Copyright NumFOCUS
diff --git a/Utilities/Maintenance/VCL_ModernizeNaming.py b/Utilities/Maintenance/VCL_ModernizeNaming.py
index 251c75cc7d1..6ba199aa5ab 100755
--- a/Utilities/Maintenance/VCL_ModernizeNaming.py
+++ b/Utilities/Maintenance/VCL_ModernizeNaming.py
@@ -1,4 +1,4 @@
-#!python
+#!/usr/bin/env python3
 # \author Hans J. Johnson
 #
 # Now that all supported compilers simply
diff --git a/Utilities/Maintenance/VNL_ModernizeNaming.py b/Utilities/Maintenance/VNL_ModernizeNaming.py
index 19dd46e64b0..d1f74727756 100755
--- a/Utilities/Maintenance/VNL_ModernizeNaming.py
+++ b/Utilities/Maintenance/VNL_ModernizeNaming.py
@@ -1,4 +1,4 @@
-#!/bin/env python
+#!/usr/bin/env python3
 # \author Hans J. Johnson
 #
 # Prepare for the future by recommending
diff --git a/Utilities/Maintenance/VerifyURLs.py b/Utilities/Maintenance/VerifyURLs.py
index ee21b38677a..5401e6e9ada 100755
--- a/Utilities/Maintenance/VerifyURLs.py
+++ b/Utilities/Maintenance/VerifyURLs.py
@@ -1,4 +1,4 @@
-#!/usr/bin/env python
+#!/usr/bin/env python3
 
 
 import sys
diff --git a/Utilities/Maintenance/VisualizeTestTimes.py b/Utilities/Maintenance/VisualizeTestTimes.py
index 7b900e87c67..13db774533f 100755
--- a/Utilities/Maintenance/VisualizeTestTimes.py
+++ b/Utilities/Maintenance/VisualizeTestTimes.py
@@ -1,4 +1,4 @@
-#!/usr/bin/env python
+#!/usr/bin/env python3
 
 """VisualizeTestTimes.py
 
diff --git a/Utilities/Maintenance/WhatModulesITK.py b/Utilities/Maintenance/WhatModulesITK.py
index c09acecf01f..ddb0fe0497c 100755
--- a/Utilities/Maintenance/WhatModulesITK.py
+++ b/Utilities/Maintenance/WhatModulesITK.py
@@ -1,4 +1,4 @@
-#!/usr/bin/env python
+#!/usr/bin/env python3
 
 
 import os, sys
diff --git a/Wrapping/Generators/Doc/doxy2swig.py b/Wrapping/Generators/Doc/doxy2swig.py
index f97072b9041..3177343cbf6 100755
--- a/Wrapping/Generators/Doc/doxy2swig.py
+++ b/Wrapping/Generators/Doc/doxy2swig.py
@@ -1,4 +1,4 @@
-#!/usr/bin/env python
+#!/usr/bin/env python3
 """Doxygen XML to SWIG docstring converter.
 
 Converts Doxygen generated XML files into a file containing docstrings
diff --git a/Wrapping/Generators/Doc/itk_doxy2swig.py b/Wrapping/Generators/Doc/itk_doxy2swig.py
index cd7d2dba942..3fa3f82278e 100755
--- a/Wrapping/Generators/Doc/itk_doxy2swig.py
+++ b/Wrapping/Generators/Doc/itk_doxy2swig.py
@@ -1,4 +1,4 @@
-#!/usr/bin/env python
+#!/usr/bin/env python3
 """Doxygen XML to SWIG docstring converter for ITK classes.
 
 Usage:
diff --git a/Wrapping/Generators/Python/Tests/multiprocess_lazy_loading.py b/Wrapping/Generators/Python/Tests/multiprocess_lazy_loading.py
index e95e8a8a213..394fdcaab79 100755
--- a/Wrapping/Generators/Python/Tests/multiprocess_lazy_loading.py
+++ b/Wrapping/Generators/Python/Tests/multiprocess_lazy_loading.py
@@ -1,4 +1,4 @@
-#!/usr/bin/env python
+#!/usr/bin/env python3
 # LazyLoading must be threadsafe
 #
 # The loading of modules in python *must* occur as a
diff --git a/Wrapping/Generators/SwigInterface/igenerator.py b/Wrapping/Generators/SwigInterface/igenerator.py
index 62b1a93a5c9..01180ff0ba0 100755
--- a/Wrapping/Generators/SwigInterface/igenerator.py
+++ b/Wrapping/Generators/SwigInterface/igenerator.py
@@ -1,4 +1,4 @@
-#!/usr/bin/env python
+#!/usr/bin/env python3
 """
 igenerator.py
 PURPOSE: