An alternative, more involved pseudobulking method would include grouping by phenotype to compare TCRs across cell types. this would include:
- mapping cell sequencing or scRNA expression data to phenotype (CD4-, CD8-, memory T cell, etc)
- grouping sequences by phenotype
- then collapsing by full nucleotide sequence to get bulk TCR sequencing per phenotype
An alternative, more involved pseudobulking method would include grouping by phenotype to compare TCRs across cell types. this would include: