diff --git a/.github/workflows/python.yml b/.github/workflows/python.yml
index 2aacd0a..c22ec97 100644
--- a/.github/workflows/python.yml
+++ b/.github/workflows/python.yml
@@ -26,7 +26,7 @@ jobs:
     - name: Set up Python
       uses: actions/setup-python@v6
       with:
-        python-version: '3.13'
+        python-version: '3.14'
     - name: Install dependencies
       run: python -m pip install pre-commit
     - name: Cache pre-commit environments
@@ -50,11 +50,11 @@ jobs:
     strategy:
       matrix:
         os: [ubuntu-latest, ubuntu-24.04-arm, macos-latest]
-        python-version: [3.9, "3.10", "3.11", "3.12", "3.13", "3.14-dev"]
+        python-version: [3.9, "3.10", "3.11", "3.12", "3.13", "3.14"]
         openslide: [system, wheel]
         include:
           - os: ubuntu-latest
-            python-version: "3.13"
+            python-version: "3.14"
             openslide: system
             sdist: sdist
     steps:
@@ -165,7 +165,7 @@ jobs:
         shell: bash
     strategy:
       matrix:
-        python-version: [3.9, "3.10", "3.11", "3.12", "3.13", "3.14-dev"]
+        python-version: [3.9, "3.10", "3.11", "3.12", "3.13", "3.14"]
         openslide: [zip, wheel]
     steps:
     - name: Check out repo
@@ -259,7 +259,7 @@ jobs:
     - name: Set up Python
       uses: actions/setup-python@v6
       with:
-        python-version: '3.13'
+        python-version: '3.14'
     - name: Install Python tools
       run: |
         python -m pip install --upgrade pip
diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml
index 93bffda..9fe75a8 100644
--- a/.pre-commit-config.yaml
+++ b/.pre-commit-config.yaml
@@ -23,19 +23,19 @@ repos:
         args: ["--py39-plus"]
 
   - repo: https://github.com/PyCQA/isort
-    rev: 6.0.1
+    rev: 6.1.0
     hooks:
       - id: isort
         name: Reorder python imports with isort
 
   - repo: https://github.com/psf/black
-    rev: 25.1.0
+    rev: 25.9.0
     hooks:
       - id: black
         name: Format python code with black
 
   - repo: https://github.com/asottile/blacken-docs
-    rev: 1.19.1
+    rev: 1.20.0
     hooks:
       - id: blacken-docs
         name: Format python code in documentation
@@ -54,7 +54,7 @@ repos:
         additional_dependencies: [flake8-bugbear, Flake8-pyproject]
 
   - repo: https://github.com/pre-commit/mirrors-mypy
-    rev: v1.17.1
+    rev: v1.18.2
     hooks:
       - id: mypy
         name: Check Python types
diff --git a/pyproject.toml b/pyproject.toml
index a76799d..d65a584 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -23,6 +23,7 @@ classifiers = [
     "Programming Language :: Python :: 3.11",
     "Programming Language :: Python :: 3.12",
     "Programming Language :: Python :: 3.13",
+    "Programming Language :: Python :: 3.14",
     "Topic :: Scientific/Engineering :: Bio-Informatics",
     "Typing :: Typed",
 ]
@@ -51,7 +52,7 @@ openslide = ["py.typed", "*.pyi"]
 
 [tool.black]
 skip-string-normalization = true
-target-version = ["py39", "py310", "py311", "py312", "py313"]
+target-version = ["py39", "py310", "py311", "py312", "py313", "py314"]
 
 # Ref: https://github.com/codespell-project/codespell#using-a-config-file
 [tool.codespell]