diff --git a/Lib/test/test_typing.py b/Lib/test/test_typing.py
index f35f864dce21e86..1543a8e71af4897 100644
--- a/Lib/test/test_typing.py
+++ b/Lib/test/test_typing.py
@@ -3888,6 +3888,39 @@ def __init__(self):
         self.assertIsInstance(B(), P)
         self.assertIsInstance(C(), P)
 
+    def test_none_on_non_callable_doesnt_defeat_the_abc_cache(self):
+        # gh-156413: a None-valued non-callable member used to be rejected by
+        # _proto_hook even though __instancecheck__ accepts it, which kept the
+        # class out of ABCMeta's cache and made every isinstance() call walk
+        # all of the protocol members again.
+        @runtime_checkable
+        class PAttr(Protocol):
+            x = 1
+
+        @runtime_checkable
+        class PProperty(Protocol):
+            @property
+            def x(self) -> int: ...
+
+        class B:
+            x = None
+
+        for P in (PAttr, PProperty):
+            with self.subTest(protocol=P.__name__):
+                self.assertIn("x", P.__non_callable_proto_members__)
+                self.assertIsInstance(B(), P)
+
+                # The first check must have cached B as a subclass of P, so
+                # the second one may not touch the members at all.
+                typing._lazy_load_getattr_static.cache_clear()
+                try:
+                    with patch.object(
+                        inspect, "getattr_static", side_effect=AssertionError
+                    ):
+                        self.assertIsInstance(B(), P)
+                finally:
+                    typing._lazy_load_getattr_static.cache_clear()
+
     def test_none_on_callable_blocks_implementation(self):
         @runtime_checkable
         class P(Protocol):
diff --git a/Lib/typing.py b/Lib/typing.py
index 65e1d1ea6be5844..1e6dbb90f63a503 100644
--- a/Lib/typing.py
+++ b/Lib/typing.py
@@ -2127,11 +2127,15 @@ def _proto_hook(cls, other):
     if not cls.__dict__.get('_is_protocol', False):
         return NotImplemented
 
+    # Setting a member to None only means "explicitly not implemented" for
+    # *callable* members; this mirrors _ProtocolMeta.__instancecheck__.
+    non_callable_members = cls.__dict__.get('__non_callable_proto_members__') or ()
     for attr in cls.__protocol_attrs__:
         for base in other.__mro__:
             # Check if the members appears in the class dictionary...
             if attr in base.__dict__:
-                if base.__dict__[attr] is None:
+                if (base.__dict__[attr] is None
+                        and attr not in non_callable_members):
                     return NotImplemented
                 break
 
diff --git a/Misc/NEWS.d/next/Library/2026-08-27-09-40-00.gh-issue-156413.Kq7Xm2.rst b/Misc/NEWS.d/next/Library/2026-08-27-09-40-00.gh-issue-156413.Kq7Xm2.rst
new file mode 100644
index 000000000000000..45b607ea47ace34
--- /dev/null
+++ b/Misc/NEWS.d/next/Library/2026-08-27-09-40-00.gh-issue-156413.Kq7Xm2.rst
@@ -0,0 +1,5 @@
+Make :func:`isinstance` checks against a :func:`runtime-checkable
+<typing.runtime_checkable>` :class:`typing.Protocol` take the cached fast path
+when the object's class sets a non-callable protocol member to ``None``. Such a
+class already passed the check, but was excluded from :class:`abc.ABCMeta`'s
+cache and so re-examined every protocol member on every call.