#features are available within https://buddy.wasko.org this software is no longer updated

For performing CRISPR in Saccharomyces cerevisiae (yeast) to edit the genome to change an individual amino acid within a desired protein/gene.

Input a yeast gene name, the amino acid number, and amino acid substituion desired and this python script finds nearby sgRNA target sites and generates oligonucleotides for cloning into the plasmid pML104 (or similar) and repair template oligonucleotide sequences necessary to generate a desired gene edit.

For plasmid information, see Laughery et al. Yeast. 2015 https://doi.org/10.1002/yea.3098. Plasmid is available on Addgene https://www.addgene.org/67638/

Full instructions are located within the Yeast CRISPR CURE documentation.

Stopped updating this python version 1 and started working with web version CRISPR Helper 2.0 (and fork CODY)