Skip to content
Draft
Show file tree
Hide file tree
Changes from all commits
Commits
File filter

Filter by extension

Filter by extension


Conversations
Failed to load comments.
Loading
Jump to
Jump to file
Failed to load files.
Loading
Diff view
Diff view
1,473 changes: 1,473 additions & 0 deletions 001933/DombeckLab/001933_demo.ipynb

Large diffs are not rendered by default.

60 changes: 60 additions & 0 deletions 001933/DombeckLab/README.md
Original file line number Diff line number Diff line change
@@ -0,0 +1,60 @@
# DANDI:001933 — Dombeck Lab Fiber Photometry Notebook

This notebook demonstrates how to access data from
**[DANDI:001933](https://dandiarchive.org/dandiset/001933)**, a dataset of
in vivo fiber photometry recordings of striatal dopamine release in awake,
head-fixed mice expressing the dopamine sensor GRAB-DA3m.

Two intersectional mouse lines targeting distinct dopamine neuron subtypes were
studied: Anxa1-iCre mice (Anxa1+, vulnerable subtype) with fiber photometry in
the dorsal lateral striatum (DLS), and Calb1-Cre mice (Calb1+, resilient subtype)
with fiber photometry in the dorsal medial striatum (DMS). Optogenetic activation
of subtype-specific dopamine neuron cell bodies in the substantia nigra pars
compacta (SNc) was achieved via Cre-dependent ChRmine expression and red-light
(635 nm) stimulation. LRRK2-WT and LRRK2-G2019S knockin mice were compared to
investigate how pathogenic LRRK2 kinase activity affects dopamine synaptic function
in a subtype-specific manner.

> **Authentication required** — DANDI:001933 is currently embargoed.
> A DANDI API key is required to stream data. Set it as an environment variable:
> ```bash
> export DANDI_API_KEY=your_key_here
> ```
> In Colab: add it via the Secrets panel (key icon on the left) under the name
> `DANDI_API_KEY`.

**Reference:** Chen, He et al. (in preparation)

---

## Notebooks

### `001933_demo.ipynb`

Streams a single session (subject 4007, Anxa-LRRK2-G2019S, 2025-08-13) from
DANDI and demonstrates:

1. NWBFile and subject metadata
2. Fiber photometry metadata (`FiberPhotometryTable`, devices, indicator) via
[ndx-fiber-photometry](https://github.com/catalystneuro/ndx-fiber-photometry)
and [ndx-ophys-devices](https://github.com/catalystneuro/ndx-ophys-devices)
3. Raw fiber photometry — full session and zoomed with stimulation epochs
4. `CommandedVoltageSeries` — LED switching square wave (data provenance)
5. Raw treadmill voltage (data provenance)
6. Processed fiber photometry — baseline-corrected traces and ΔF/F
7. ΔF/F aligned to a single stimulation onset
8. Treadmill behavior (velocity and acceleration)
9. Optogenetic epochs table — per-epoch power values and full-session power map
10. Optogenetics metadata via [ndx-optogenetics](https://github.com/catalystneuro/ndx-optogenetics)
11. Peri-stimulus ΔF/F (PSTH) — grand average, per-power traces, single-trial heatmap

---

## Installing the dependencies

```bash
git clone https://github.com/dandi/example-notebooks
cd example-notebooks/001933/DombeckLab
conda env create --file environment.yml
conda activate dombeck_lab_001933
```
18 changes: 18 additions & 0 deletions 001933/DombeckLab/environment.yml
Original file line number Diff line number Diff line change
@@ -0,0 +1,18 @@
name: dombeck_lab_001933
channels:
- conda-forge
dependencies:
- python==3.12
- pip
- pip:
- dandi
- jupyter
- h5py
- remfile
- pynwb
- numpy
- pandas
- matplotlib
- ndx-fiber-photometry>=0.2.3
- ndx-ophys-devices>=0.3.1
- ndx-optogenetics>=0.4.0
Loading